Plant-beneficial Bacillus, Pseudomonas, and Staphylococcus spp. from Kumaon Himalayas and their drought tolerance response

Plant growth-promoting rhizobacteria (PGPR) have been shown to augment plant responses against drought and other abiotic stresses. In the present study, we isolated 27 bacteria from rhizosphere of various plants cultivated in Kumaon Himalayas., measure their stress tolerance, these isolates were subjected variations pH, temperature, drought. All also screened for traits. Among these, four RR1, ASC1, AFS3, NG4 demonstrated growth promotion activities including synthesis indole-3-acetic acid (IAA), siderophores, ammonia, 1-aminocyclopropane-1-carboxylic (ACC) deaminase production, concomitantly high tolerance Moreover, 16S rRNA sequencing validated identities as Bacillus, Pseudomonas , Staphylococcus sp. Finally, assess in-vivo potential isolates, a pot-trial experiment was undertaken wheat cultivar VL-892. The results that inoculating with PGPR greatly improved under circumstances by increasing root shoot length both fresh dry weight shoot. This study endeavors discover biochemical molecular diversity cultivable six remotely located districts Uttarakhand. conclusion, drought-tolerant strains described this are plant-beneficial can effectively mobilize nutrients conditions. Consequently, they could be used bioinoculants alleviate plants, sustainable manner. To best our knowledge, is first report exploring characterization Himalayas evaluation.